Fig. 4. Assessment of CXCL14 interactions with CXCR4 and CXCR7 receptors. A) mRNA profiling of Cxc-family receptors by mouse islets and MIN6 b-cells. Data are expressed as mean+SEM expression relative to Actb, Gapdh, Ppia, Tbp and Tfrc, n=4. T: trace expression; A: absent expression. B,C) CXCL12 induced b-arrestin recruitment at the mouse CXCR4 (B) and CXCR7 (C) receptors, with EC₅₀ values of 15.86ng/mL and 31.29ng/mL and Hill slope values of 1.079 and 1.134, respectively. CXCL14 did not promote CXCR4 or CXCR7 b-arrestin recruitment. Data are expressed as mean±range, n=2. D,E) Increasing concentrations of CXCL14 did not significantly affect CXCL12 concentration-response profiles at CXCR4 (D) or CXCR7 (E) receptors. Data are expressed as mean±range, n=2.